Other

Part:BBa_K2149020:Design

Designed by: Aline Larissa Gonçalves   Group: iGEM16_USP-EEL-Brazil   (2016-10-19)


RFP with Lux D and an aldehyde decarbonylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1897
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1285
    Illegal BamHI site found at 1678
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1719


Design Notes

The sequence of an aldehyde decarbonylase was synthesized after the gene Lux D with a RBS and a non-specific region between then.


Source

This sequence was taken from the cyanobacterium Synechococcus elongatus used in the work "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli" of Dr. Howard and collaborators for the production of alkanes.

References

HOWARD, T. P. et al. Synthesis of customized petroleum-replica fuel molecules by targeted modi fi cation of free fatty acid pools in Escherichia coli. PNAS, p. 1–6, 2013.